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author:

Qiu, Bin (Qiu, Bin.) [1] (Scholars:邱彬) | Zhang, Ya-shan (Zhang, Ya-shan.) [2] | Lin, Yi-bing (Lin, Yi-bing.) [3] | Lu, Yu-Jing (Lu, Yu-Jing.) [4] | Lin, Zhen-yu (Lin, Zhen-yu.) [5] (Scholars:林振宇) | Wong, Kwok-Yin (Wong, Kwok-Yin.) [6] | Chen, Guo-nan (Chen, Guo-nan.) [7] (Scholars:陈国南)

Indexed by:

EI Scopus SCIE

Abstract:

In this paper, we reported a convenient fluorescence method for the detection of genetically modified organisms (GMOs). As it is known that the cauliflower mosaic virus (CaMV) 35S promoter is widely used in most transgenic plants (Schnurr and Guerra, 2000), we thus design a simple method based on the detection of a section target DNA (DNA-T) from the transgene CaMV 35S promoter. In this method, the full-length guanine-rich single-strand sequences were split into fragments (Probe 1 and 2) and each part of the fragment possesses two GGG repeats. In the presence of K+ ion and berberine, if a complementary target DNA of the CaMV 35S promoter was introduced to hybridize with Probe 1 and 2, a G-quadruplex-berberine complex was thus formed and generated a strong fluorescence signal. The generation of fluorescence signal indicates the presence of CaMV 35S promoter. This method is able to identify and quantify Genetically Modified Organisms (GMOs), and it shows wide linear ranges from 5.0 x 10(-9) to 9.0 x 10(-7) mol/L with a detection limit of 2.0 x 10(-9) mol/L. (C) 2012 Elsevier B.V. All rights reserved.

Keyword:

Berberine Cauliflower mosaic virus 35S Fluorescent biosensor Genetically modified organisms G-quadruplex

Community:

  • [ 1 ] [Qiu, Bin]Fuzhou Univ, Dept Chem, Minist Educ, Key Lab Anal & Detect Technol Food Safety Fuzhou, Fuzhou 350002, Peoples R China
  • [ 2 ] [Zhang, Ya-shan]Fuzhou Univ, Dept Chem, Minist Educ, Key Lab Anal & Detect Technol Food Safety Fuzhou, Fuzhou 350002, Peoples R China
  • [ 3 ] [Lin, Yi-bing]Fuzhou Univ, Dept Chem, Minist Educ, Key Lab Anal & Detect Technol Food Safety Fuzhou, Fuzhou 350002, Peoples R China
  • [ 4 ] [Lu, Yu-Jing]Fuzhou Univ, Dept Chem, Minist Educ, Key Lab Anal & Detect Technol Food Safety Fuzhou, Fuzhou 350002, Peoples R China
  • [ 5 ] [Lin, Zhen-yu]Fuzhou Univ, Dept Chem, Minist Educ, Key Lab Anal & Detect Technol Food Safety Fuzhou, Fuzhou 350002, Peoples R China
  • [ 6 ] [Chen, Guo-nan]Fuzhou Univ, Dept Chem, Minist Educ, Key Lab Anal & Detect Technol Food Safety Fuzhou, Fuzhou 350002, Peoples R China
  • [ 7 ] [Qiu, Bin]Hong Kong Polytech Univ, Dept Appl Biol & Chem Technol, State Key Lab Chirosci, Kowloon, Hong Kong, Peoples R China
  • [ 8 ] [Wong, Kwok-Yin]Hong Kong Polytech Univ, Dept Appl Biol & Chem Technol, State Key Lab Chirosci, Kowloon, Hong Kong, Peoples R China
  • [ 9 ] [Lu, Yu-Jing]Guangdong Univ Technol, Fac Chem Engn & Light Ind, Guangzhou 510006, Guangdong, Peoples R China

Reprint 's Address:

  • 陈国南

    [Chen, Guo-nan]Fuzhou Univ, Dept Chem, Minist Educ, Key Lab Anal & Detect Technol Food Safety Fuzhou, Fuzhou 350002, Peoples R China

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Source :

BIOSENSORS & BIOELECTRONICS

ISSN: 0956-5663

Year: 2013

Volume: 41

Page: 168-171

6 . 4 5 1

JCR@2013

1 0 . 7 0 0

JCR@2023

ESI Discipline: CHEMISTRY;

JCR Journal Grade:1

CAS Journal Grade:1

Cited Count:

WoS CC Cited Count: 24

SCOPUS Cited Count: 26

ESI Highly Cited Papers on the List: 0 Unfold All

WanFang Cited Count:

Chinese Cited Count:

30 Days PV: 0

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