Click　chemistry,　a　new　molecular　approach　proposed　by　Sharpless　and　co-workers　in　2001,　is　the　most　practical　and　reliable　chemical　reactions　to　connect　a　diversity　of　structures.　The　Cu　(I)　catalyzed　azide-alkyne　1,3-dipolar　cycloaddition　is　one　of　the　most　common　reactions　in　click　chemistry,　which　has　high　yield,　good　efficiency,　and　high　purity.　In　addition,　this　click　reaction　is　essentially　inert　to　most　biological　molecules,　oxygen,　water,　and　tolerant　of　a　wide　range　of　solvents,　pH　values　and　temperatures,　and　allows　mild　reaction　conditions.　This　click　reaction　also　has　excellent　selectivity　in　chemical　synthesis.　Owing　to　the　fact　that　the　azide　and　alkyne　groups　are　facile　introduced　in　the　structure　of　molecules　and　form　a　stable　1,　2,3-trizoles　via　click　reaction,　it　has　been　widely　applied　in　syntheses　of　functional　polymers　and　biomacromolecules,　surfaces　modification,　functionalization　of　DNAs　and　carbon　nanotubes,　and　it　has　been　also　widely　used　for　fabrication　of　biological　or　chemical　sensors.　In　recent　years,　the　cells　and　viruses　have　been　modified　by　using　click　chemistry,　while　it　doesn＇t　damage　the　cells　and　viruses,　and　still　keep　their　good　biological　activity.　In　this　review,　the　basic　principle,　characteristics　and　advantages　of　click　chemistry　are　described,　the　recent　progress　and　some　important　research　results　in　click　chemistry　are　then　introduced,　and　finally,　the　advances　of　click　chemistry　are　prospected.