Molecularly　imprinted　polymer　(MIP)　monoliths　with　(S)-ornidazole　((S)-ONZ)　as　the　template　molecule　have　been　designed　and　prepared　by　the　simple　thermal　polymerization　of　methacrylic　acid,　4-vinylpyridine,　and　ethylene　dimethacrylate　in　the　presence　of　a　binary　porogenic　mixture　of　toluene　and　dodecanol.　The　influences　of　polymerization　mixture　composition　on　the　chiral　recognition　of　ONZ　have　been　evaluated,　and　the　imprint　effect　in　the　optimized　MIP　monolith　has　been　clearly　demonstrated.　The　new　monolithic　stationary　phase　with　optimized　porous　property　and　good　selectivity　was　used　for　the　chiral　separation　of　ONZ　by　pressurized　CEC.　The　pressurized　CEC　conditions　were　also　optimized　to　obtain　the　good　chiral　separation.　The　enantiomers　were　rapidly　separated　within　9　min　on　the　MIP-based　chiral　stationary　phase,　whereas　the　chiral　separation　was　not　obtained　on　the　nonimprinted　polymer.　Additionally,　the　proposed　method　has　been　successfully　applied　to　the　chiral　separation　of　ONZ　in　tablet　samples　by　injection　of　the　crude　sample.　The　cross-selectivity　for　similar　antiparasitic　drug　was　investigated.　The　results　indicated　that　the　chiral　separation　of　secnidazole　could　also　be　obtained　on　the　optimized　MIP　monolith　within　14　min.