An　integrated　automatic　electrochemical　immunosensor　array　has　been　designed　for　the　simultaneous　detection　of　5-type　hepatitis　virus　antigens　(i.e.　hepatitis　A,　hepatitis　B,　hepatitis　C,　hepatitis　D,　and　hepatitis　E).　Initially,　5-type　hepatitis　virus　antibodies　were　immobilized　onto　a　self-made　electrochemical　sensor　array　using　nanogold　particles　and　protein　A　as　matrices,　and　the　immunosensor　array　was　then　used　to　capture　their　corresponding　antigens　from　sample　solution　with　a　1-step　capture　format.　The　detection　is　based　on　the　potential　change　before　and　after　the　antigen-antibody　reaction　by　using　a　2-electrode　system.　The　developed　immunosensor　array　with　allowed　simultaneous　determination　of　5-type　hepatitis　virus　antigens　in　5　min.　The　detection　limit　of　the　sensor　array　was　＜=　1.0　ng/mL　for　most　analytes.　The　intra-　and　inter-assay　imprecision　(CVs)　of　the　sensor　array　were　＜=　7.3%　and　8.1%,　respectively.　No　nonspecific　adsorption　was　evaluated,　and　the　crossing　talk　between　neighboring　sites　was　＜=　7.5%　(CV).　The　developed　immunoassay　system　offers　promise　for　label-free,　simple,　rapid,　cost-effective　analysis　of　multi-analyte.　Importantly,　the　chip-based　immunosensor　array　could　open　new　opportunities　for　high-throughput　multi-analyte　immunoassays　in　point-of-care　testing　of　miniaturized　lab-on-a-chip　devices.　(C)　2009　Elsevier　B.V.　All　rights　reserved.