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author:

Li, Tianlin (Li, Tianlin.) [1] | Zhang, Zhuomin (Zhang, Zhuomin.) [2] | Zhang, Lan (Zhang, Lan.) [3] (Scholars:张兰) | Huang, Xinjian (Huang, Xinjian.) [4] | Lin, Junwei (Lin, Junwei.) [5] | Chen, Guonan (Chen, Guonan.) [6] (Scholars:陈国南)

Indexed by:

EI Scopus SCIE

Abstract:

An improved fast method for extraction of steroidal saponins in Tribulus terrestris based on the use of focus microwave-assisted extraction (FMAE) is proposed. Under optimized conditions, four steroidal saponins were extracted from Tribulus terrestris and identified by GC-MS, which are Tigogenin (TG), Gitogenin (GG), Hecogenin (HG) and Neohecogenin (NG). One of the most important steroidal saponins, namely TG was quantified finally. The recovery of TG was in the range of 86.7-91.9% with RSD < 5.2%. The conventional heating reflux extraction was also conducted in order to validate the reliability of this new FMAE method. The yield of total steroidal saponins was 90.3% in a one-step FMAE, while the yield of 65.0% was achieved during heating reflux extraction, and the extraction time was reduced from 3 h to 5 min by using less solvent. The method was successfully applied to analyze the steroidal saponins of Tribulus terrestris from different areas of occurrence. The difference in chromatographic characteristics of steroidal saponins was proved to be related to the different areas of occurrence. The results showed that FMAE-GC-MS is a simple, rapid, solvent-saving method for the extraction and determination of steroidal saponins in Tribulus terrestris.

Keyword:

Focus microwave-assisted extraction GC-MS Steroidal saponins Tribulus terrestris

Community:

  • [ 1 ] [Li, Tianlin]Fuzhou Univ, Minist Educ, Key Lab Anal & Detect Food Safety, Fuzhou 350002, Fujian, Peoples R China
  • [ 2 ] [Zhang, Zhuomin]Fuzhou Univ, Minist Educ, Key Lab Anal & Detect Food Safety, Fuzhou 350002, Fujian, Peoples R China
  • [ 3 ] [Zhang, Lan]Fuzhou Univ, Minist Educ, Key Lab Anal & Detect Food Safety, Fuzhou 350002, Fujian, Peoples R China
  • [ 4 ] [Chen, Guonan]Fuzhou Univ, Minist Educ, Key Lab Anal & Detect Food Safety, Fuzhou 350002, Fujian, Peoples R China
  • [ 5 ] [Li, Tianlin]Fuzhou Univ, Dept Chem, Fuzhou 350002, Fujian, Peoples R China
  • [ 6 ] [Zhang, Zhuomin]Fuzhou Univ, Dept Chem, Fuzhou 350002, Fujian, Peoples R China
  • [ 7 ] [Zhang, Lan]Fuzhou Univ, Dept Chem, Fuzhou 350002, Fujian, Peoples R China
  • [ 8 ] [Huang, Xinjian]Fuzhou Univ, Dept Chem, Fuzhou 350002, Fujian, Peoples R China
  • [ 9 ] [Lin, Junwei]Fuzhou Univ, Dept Chem, Fuzhou 350002, Fujian, Peoples R China

Reprint 's Address:

  • 张兰

    [Zhang, Lan]Fuzhou Univ, Minist Educ, Key Lab Anal & Detect Food Safety, Fuzhou 350002, Fujian, Peoples R China

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Source :

JOURNAL OF SEPARATION SCIENCE

ISSN: 1615-9306

Year: 2009

Issue: 23-24

Volume: 32

Page: 4167-4175

2 . 5 5 1

JCR@2009

2 . 8 0 0

JCR@2023

ESI Discipline: CHEMISTRY;

JCR Journal Grade:2

CAS Journal Grade:1

Cited Count:

WoS CC Cited Count: 17

SCOPUS Cited Count: 24

ESI Highly Cited Papers on the List: 0 Unfold All

WanFang Cited Count:

Chinese Cited Count:

30 Days PV: 3

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