A　novel　polymethacrylate-based　monolithic　column　with　covalently　bonded　zwitterionic　functional　groups　was　prepared　by　in　situ　copolymerization　of　N.N-dimethyl-N-methacryloxyethyl　N-(3-sulfopropyl)　ammonium　betaine　(SPE),　pentaerythritol　triacrylate　(PETA),　and　vinylsulfonic　acid　(VS)　in　a　binary　porogenic　solvent　consisting　of　cyclohexanol　and　ethylene　glycol.　This　monolith　was　developed　as　a　separation　column　for　CEC.　While　SPE　functioned　as　both　an　electrostatic　interaction　stationary　phase　and　the　polar　ligand　provider,　VS　was　employed　to　generate　EOF.　PETA,　which　has　much　more　hydrophilicity　due　to　a　hydroxyl　sub-layer,　was　used　to　replace　ethylene　dimethacrylate　as　a　cross-linker.　The　monolith　provided　an　adequate　EOF　when　VS　level　was　maintained　at　0.6%　w/w.　Different　monolithic　stationary　phases　were　easily　prepared　by　adjusting　the　ratio　of　PETA/SPE　in　the　polymerization　solution　as　well　as　the　composition　of　the　porogenic　solvent.　The　observed　RSD　were　＜=　3.6,　＜=　4.3　and　＜=　5.6%　for　the　EOF　velocity,　retention　time,　and　column　efficiency,　respectively.　The　column　efficiencies　greater　than　145　000　theoretical　plates/m　for　thiourea　and　132　000　theoretical　plates/m　for　charged　cytidine　were　obtained.　The　poly(SPE-co-PETA-co-VS)　monolith　showed　good　selectivity　for　neutral　and　charged　polar　analytes.　It　was　found　that　the　separation　mechanism　of　charged　polar　solutes　was　attributed　to　a　mixed　mode　of　hydrophilic　interaction　and　electrostatic　interaction,　as　well　as　electrophoresis.　No　peak　tailing　was　observed　for　the　separation　of　basic　compounds,　such　as　basic　nucleic　acid　bases　and　nucleoside　on　the　monolith.