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author:

Guo, Longhua (Guo, Longhua.) [1] | Qiu, Bin (Qiu, Bin.) [2] (Scholars:邱彬) | Chi, Yuwu (Chi, Yuwu.) [3] (Scholars:池毓务) | Chen, Guonan (Chen, Guonan.) [4] (Scholars:陈国南)

Indexed by:

Scopus SCIE

Abstract:

In this paper, an ultrasensitive CE-CL detection system coupled with a novel double-on-column coaxial flow detection interface was developed for the detection of PCR products. A reliable procedure based on this system had been demonstrated for qualitative and quantitative analysis of genetically modified organism-the detection of Roundup Ready Soy (RRS) samples was presented as an example. The promoter, terminator, function and two reference genes of RRS were amplified with multiplex PCR simultaneously. After that, the multiplex PCR products were labeled with acridinium ester at the 5'-terminal through an amino modification and then analyzed by the proposed CE-CL system. Reproducibility of analysis times and peak heights for the CE-CL analysis were determined to be better than 0.91 and 3.07% (RSD, n = 15), respectively, for three consecutive days. It was shown that this method could accurately and qualitatively detect RRS standards and the simulative samples. The evaluation in terms of quantitative analysis of RRS provided by this new method was confirmed by comparing our assay results with those of the standard real-time quantitative PCR (RT-QPCR) using SYBR (R) Green I dyes. The results showed a good coherence between the two methods. This approach demonstrated the possibility for accurate qualitative and quantitative detection of GM plants in a single run.

Keyword:

Capillary electrophoresis Chemiluminescent detection Genetically modified organism PCR

Community:

  • [ 1 ] [Guo, Longhua]Fuzhou Univ, Dept Chem, Fuzhou 350002, Fujian, Peoples R China
  • [ 2 ] [Qiu, Bin]Fuzhou Univ, Dept Chem, Fuzhou 350002, Fujian, Peoples R China
  • [ 3 ] [Chi, Yuwu]Fuzhou Univ, Dept Chem, Fuzhou 350002, Fujian, Peoples R China
  • [ 4 ] [Chen, Guonan]Fuzhou Univ, Dept Chem, Fuzhou 350002, Fujian, Peoples R China
  • [ 5 ] [Guo, Longhua]Fuzhou Univ, Minist Educ, Key Lab Anal & Detect Technol Food Safety, Fuzhou 350002, Fujian, Peoples R China
  • [ 6 ] [Qiu, Bin]Fuzhou Univ, Minist Educ, Key Lab Anal & Detect Technol Food Safety, Fuzhou 350002, Fujian, Peoples R China
  • [ 7 ] [Chi, Yuwu]Fuzhou Univ, Minist Educ, Key Lab Anal & Detect Technol Food Safety, Fuzhou 350002, Fujian, Peoples R China
  • [ 8 ] [Chen, Guonan]Fuzhou Univ, Minist Educ, Key Lab Anal & Detect Technol Food Safety, Fuzhou 350002, Fujian, Peoples R China

Reprint 's Address:

  • 陈国南

    [Chen, Guonan]Fuzhou Univ, Dept Chem, Fuzhou 350002, Fujian, Peoples R China

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Source :

ELECTROPHORESIS

ISSN: 0173-0835

Year: 2008

Issue: 18

Volume: 29

Page: 3801-3809

3 . 5 0 9

JCR@2008

3 . 0 0 0

JCR@2023

ESI Discipline: CHEMISTRY;

JCR Journal Grade:1

Cited Count:

WoS CC Cited Count: 15

SCOPUS Cited Count: 18

ESI Highly Cited Papers on the List: 0 Unfold All

WanFang Cited Count:

Chinese Cited Count:

30 Days PV: 2

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