A　new,　simple,　and　fast　method　to　integrate　indium　tin　oxide　electrode　in　an　amperometric　detection　(AD)　microchip　is　introduced.　Without　the　help　of　photoresist　and　complicated　apparatus,　the　microchip　could　be　fabricated　in　most　laboratories　in　a　very　short　time　by　this　method.　The　experiment　indicated　that　the　microchip　was　stable　and　had　good　reproducibility.　On　this　microchip,　a　new　method　was　established　to　separate　and　determine　synephrine　and　hesperidin,　which　are　the　main　electroactively　bioactive　ingredients　of　pericarpium　citri　reticulatae,　by　AD.　Under　the　optimal　conditions,　the　two　compounds　could　be　completely　separated　within　5.5　min　and　the　detection　limits　were　0.13　and　0.57　mu　g/mL,　respectively.　The　proposed　method　has　been　successfully　used　to　determine　synephrine　and　hesperidin　in　real　pericarpium　citri　reticulatae　sample,　and　the　results　show　that　the　proposed　method　is　sensitive,　reliable,　fast,　and　economical.