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author:

Lin, XC (Lin, XC.) [1] | Xie, ZH (Xie, ZH.) [2] (Scholars:谢增鸿) | Guo, LQ (Guo, LQ.) [3] (Scholars:郭良洽) | Chen, GN (Chen, GN.) [4] (Scholars:陈国南)

Indexed by:

SCIE PKU CSCD

Abstract:

A fluorescence-enhanced system was developed for the determination of nucleic acids by using morin-aluminum(III) complex as a new fluorescent probe. In aqueous solution, morin-aluminum(III) complex showed maximum excitation and emission wavelengths at 420.0 nm and 532.8 nm, respectively, and its fluorescence could be greatly enhanced in the presence of nucleic acids. Under optimal conditions, the calibration graph was linear over the range 0.25-1.50 mug(.)mL(-1) for fish sperm DNA, 0.10-1.60 mug(.)mL(-1) for salmon sperm DNA, 0.25-2.00 mug(.)mL(-1) for calf thymus DNA and 0.25-2.00 mug(.)mL(-1) for yeast RNA. The corresponding detection limits are 3 ng(.)mL(-1), 2 ng(.)mL(-1), 2 ng(.)mL(-1) and 3 ng(.)mL(-1), respectively. Applied for the determination of nucleic acids in synthetic samples, the relative standard deviation for five replicates is less than 3.6%, and the recovery ranges from 93.3% to 107.9%. Additionally, the interaction mechanism of morin-aluminum(III) with nucleic acids is also discussed.

Keyword:

fluorescent robe morin-aluminum(III) nucleic acids

Community:

  • [ 1 ] Fuzhou Univ, Dept Chem, Fujian 350002, Peoples R China

Reprint 's Address:

  • 林旭聪

    [Lin, XC]Fuzhou Univ, Dept Chem, Fujian 350002, Peoples R China

Email:

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Source :

SPECTROSCOPY AND SPECTRAL ANALYSIS

ISSN: 1000-0593

CN: 11-2200/O4

Year: 2004

Issue: 10

Volume: 24

Page: 1230-1234

0 . 3 5

JCR@2004

0 . 7 0 0

JCR@2023

ESI Discipline: CHEMISTRY;

JCR Journal Grade:4

Cited Count:

WoS CC Cited Count: 4

SCOPUS Cited Count:

ESI Highly Cited Papers on the List: 0 Unfold All

WanFang Cited Count:

Chinese Cited Count:

30 Days PV: 2

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