Exosomes　are　small-sized　extracellular　vesicles　that　are　released　by　many　cell　types　and　can　be　found　in　different　bodily　fluids.　Exosomes　have　attracted　significant　attention　over　the　past　decades　as　they　contain　biomolecules　with　a　similar　profile　to　their　cell　of　origin　that　can　be　used　in　identifying　normal　and　pathological　cell　states,　such　as　lipids,　nucleic　acids,　and　proteins.　Therefore,　exosomes　are　being　valued　over　their　diagnostic　and　therapeutic　utilities　and　their　potential　as　novel　drug　delivery　vehicles.　Despite　their　usefulness,　exosomes　are　approximately　30-200　nm　and　can　vary　in　contents　and　compositions,　thus　making　the　separation　and　detection　of　them　extremely　challenging.　Current　exosome　isolation　and　detection　techniques　are　limited　by　low　yield,　low　purity,　requiring　cumbersome　instruments,　and　relatively　long　processing　time.　However,　recent　advancements　of　microfluidics　technologies　offered　new　exosome　isolation　and　detection　techniques　with　the　great　potential　to　overcome　these　limitations.　Here,　we　compiled　a　thorough　review　that　includes　latest　works　on　using　microfluidic　technologies　to　isolate　and　detect　exosomes　in　different　bodily　fluids　and　comments　on　their　advantages　and　disadvantages,　with　the　hope　to　identify　future　research　opportunities　and　direction　in　the　field.　©　2013　IEEE.