Aim　To　evaluate　the　inhibitive　effects　of　celastrol　on　LDL　oxidation　and　HAEC　cell　oxidative　damage.　Methods　The　Cu2　+-induced　LDL　oxidation　model　was　employed　to　evaluate　celastrol　inhibitive　effect　on　LDL　oxidation　in　vitro,　the　oxidative　reaction　kinetic　curves　were　determined,　and　the　AUC,　lag　time,TBARS　value　were　assayed.　The　AAPH-induced　HAEC　damaging　cellular　model　was　employed　to　evaluate　the　effect　of　celastrol　on　oxidative　cellular　damage.　The　safe　dose　of　celastrol　on　normal　cells　was　determined　by　MTT　method,　and　the　effects　of　celastrol　on　HAEC　oxidative　damage　were　evaluated　at　the　range　of　this　safe　dose.　The　LDH　leakage,　ROS　level,　SOD　and　GPX　enzymatic　activity,　Nrf2　and　HO-1　mRNA　expression　were　determined.　Results　At　the　dose　range　of　100　nmol　·　L-1　to　1　μmol　·　L-1,　celastrol　effectively　extended　the　lag　time　of　LDL　oxidative　process　induced　by　Cu2+,　reduced　the　AUC　of　oxidative　reaction　kinetic　curve　and　reduced　the　generation　of　lipid　peroxide　in　the　LDL　oxidative　process.　In　the　cellular　experiment,　celastrol　effectively　reduced　the　LDH　leakage　induced　by　AAPH,　increased　the　integrity　of　cell　membrane　and　nucleus,　enhanced　the　antioxidative　enzyme　activities　of　cellular　SOD,GPX　and　increased　the　expressions　of　Nrf2,　HO-1　mRNA,　celastrol　also　maintained　the　integrity　of　cellular　structure.　Conlusion　Celastrol　can　effectively　inhibit　LDL　oxidation　induced　by　Cu2+,　and　can　inhibit　HAEC　cell　oxidative　damage　induced　by　AAPH　at　the　dose　range　of　100　to　400　nmol　·　L-1.