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author:

严芬 (严芬.) [1] | 连燕萍 (连燕萍.) [2] | 杨光 (杨光.) [3] | 王培松 (王培松.) [4] | 吴晨烁 (吴晨烁.) [5] | 陈宁辛 (陈宁辛.) [6]

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CQVIP PKU CSCD

Abstract:

采用透明圈初筛方法得到一株产褐藻胶裂解酶菌株B1,通过形态观察和16S r DNA序列分析鉴定该菌株为假交替单胞菌属(Pseudoalteromonas sp.)。通过单因素实验对菌株B1产酶条件进行优化,最佳培养基组成为:褐藻酸钠1%,氯化铵0.2%,Na Cl 3%,KH2PO40.02%,Mg SO40.01%,Ca Cl2·2H2O 0.1%,初始p H5.5。最佳的培养条件:装液量75 m L,培养温度30℃,摇床转速180 r/min,培养22 h。在该条件下,褐藻胶裂解酶最高酶活力提高到71.94 U/m L,提高了72.11%。

Keyword:

假交替单胞菌 培养条件 筛选 褐藻胶裂解酶 鉴定

Community:

  • [ 1 ] 福州大学生物科学与工程学院,福建福州350116

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Source :

食品工业科技

ISSN: 1002-0306

Year: 2015

Issue: 22

Volume: 36

Page: 287-292

Cited Count:

WoS CC Cited Count: 0

SCOPUS Cited Count:

ESI Highly Cited Papers on the List: 0 Unfold All

WanFang Cited Count:

Chinese Cited Count: -1

30 Days PV: 5

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