The　aim　of　present　study　was　to　investigate　the　regulatory　effects　of　an　extract　of　the　Grifola　frondosa　on　glycolipid　metabolism　and　the　intestinal　flora　in　high-fat　diet　fed　rats.　The　ethyl　acetate　extract　of　G.　frondosa　(EAE),　enriched　with　pseudobaptigenin　and　cyanidin　3-O-xylosylrutinoside,　exerted　a　stronger　inhibitory　effect　on　lipase　than　the　other　polar　fractions　of　G.　frondosa　in　vitro.　Supplementation　with　EAE　(50　mg/kg)　to　rats　for　8　weeks　clearly　ameliorated　dyslipidaemia　by　suppressing　the　serum　levels　of　glucose　(Glu),　total　triglycerides　(TG)　and　low-density　lipoprotein　cholesterol　(LDL-C),　and　hepatic　fat　accumulation.　Furthermore,　EAE　supplementation　normalized　the　intestinal　flora　structure　in　high-fat　diet　fed　rats.　The　relevance　analysis　suggested　strong　associations　of　Anaerotruncus,　Barnesiella,　Clostridiaceae,　Erysipelotrichaceae,　Paraprevotella,　Clostridium_XVIII,　Coprococcus　and　Turicibacter　with　the　biochemical　indexes.　Simultaneously,　the　EAE　treatment　intervened　the　expression　levels　of　the　genes　associated　with　glycolipid　metabolism.　Our　results　indicated　that　pseudobaptigenin　and　cyanidin-3-O-xylosylrutinoside　can　be　used　as　pharmaceutical　agents　for　the　prevention　of　the　disorder　of　glycolipid　metabolism.