Persistent　luminescent　nanoparticles　(PLNPs)　are　a　class　of　materials　with　excellent　optical　properties,　which　can　continue　to　emit　light　for　a　long　time　after　removing　the　excitation　light　source.　This　feature　enables　PLNPs　to　be　used　for　development　of　biological　detection　modes　without　autofluorescence　background.　In　this　study,　we　prepared　Zn2GeO4:　Mn2+,　Pr3+　(ZGOMP)　nanorods　through　a　one-pot　hydrothermal　method.　Using　the　pH-responsive　luminescence　behavior　of　ZGOMP,　we　developed　an　autofluorescence-free　biosensor　using　ZGOMP　as　a　probe　and　gluconic　acid　as　a　quencher　to　detect　prostate-specific　antigen　(PSA).　Hybridization　chain　reaction　(HCR)　and　magnetic　separation　system　were　introduced　in　the　design　to　achieve　efficient　signal　amplification.　Under　the　optimal　conditions,　the　as-designed　autofluorescence-free　sensing　platform　showed　high　selectivity,　and　showed　a　good　luminescence　response　to　PSA　within　the　linear　range　of　0.001-10　ng/mL　at　a　detection　limit　of　0.64　pg/mL.　The　excellent　analytical　performance　shows　that　the　current　strategy　provides　an　effective　platform　for　clinical　sample　analysis.