Methods　based　on　immunoassays　have　been　developed　for　cancer　biomarker　alpha-fetoprotein　(AFP),　but　most　involve　complicated　or　stripping　procedures　and　are　unfavourable　for　routine　use.　Herein　we　report　the　proof-of-concept　of　simple　and　point-of-care　(POC)　immunoassay　for　AFP　in　hepatocellular　carcinoma　on　a　portable　personal　glucometer　(PGM)　by　using　antibody-invertase　cross-linkage　nanoparticles　as　the　signal-generation　tags.　Antibody-invertase　cross-linkage　nano　tags　were　synthesized　by　using　reverse　micellar　method　with　glutaraldehyde.　The　POC　immunoassay　was　carried　out　on　monoclonal　anti-AFP　capture　antibody-coated　microplate　with　a　sandwich-type　reaction　mode.　Introduction　of　invertase　nano　labels　accompanying　target　AFP　could　hydrolyse　sucrose　into　glucose　and　fructose.　The　produced　glucose　molecules　could　be　determined　on　a　portable　personal　glucometer.　Relative　to　unimolecular　invertase　labelling,　improved　analytical　features　were　acquired　with　antibody-invertase　nano　labelling.　With　the　nano　labels,　PGM-based　immunoassay　exhibited　good　electrochemical　responses　for　the　detection　of　AFP,　and　allowed　detection　of　AFP　at　a　concentration　as　low　as　5.4　pg　mL(-1).　Moreover,　a　good　repeatability　and　intermediate　precision　could　be　found　down　to　12.68　%.　Good　well-matched　results　were　obtained　for　analysis　of　human　serum　specimens　between　POC　immunoassay　and　commercial　human　AFP　ELISA　kit.