The　emergence　of　continuous-flow　microfluidics　has　led　to　a　revolution　in　biochemistry　and　biomedicine.　On　such　a　microscale　lab-on-a-chip　system,　complex　biochemical　assays,　e.g.,　DNA　analysis　and　drug　discovery,　can　be　executed　efficiently　without　any　human　intervention.　Owing　to　the　high　complexity　of　chip　architecture　and　assay　protocol,　considerable　effort　has　been　directed　towards　the　design　automation　of　such　chips　over　the　past　decade.　Existing　methods,　however,　perform　the　corresponding　design　tasks　including　binding,　scheduling,　placement,　and　routing　separately,　leading　to　serious　gaps　between　different　steps　and　may　even　cause　design　failure.　To　overcome　these　drawbacks,　in　this　paper,　we　propose　a　one-pass　architecture　synthesis　flow　called　BigIntegr,　for　continuous-flow　microfluidic　lab-on-a-chip,　integrating　all　the　design　steps　into　an　＇organic　whole＇,　which　has　never　been　considered　in　prior　work.　With　the　proposed　BigIntegr,　the　aforementioned　design　tasks　can　be　synchronized　seamlessly　and　performed　in　a　combined　manner,　thereby　eliminating　the　gaps　between　design　steps.　As　a　result,　biochip　architectures　with　both　high　efficiency　and　low　cost　can　be　generated　without　any　design　adjustments　and　modifications.　Experimental　results　on　multiple　benchmarks　demonstrate　the　effectiveness　of　the　proposed　automation　flow.　©　2021　IEEE.