The　potential　effects　of　Auricularia　auricula　melanin　(AAM)　on　the　intestinal　flora　and　liver　metabolome　in　mice　exposed　to　alcohol　intake　were　investigated　for　the　first　time.　The　results　showed　that　oral　administration　of　AAM　significantly　reduced　the　abnormal　elevation　of　serum　total　triglyceride　(TG),　cholesterol　(TC),　low　density　lipoprotein　cholesterol　(LDL-C),　aspartate　aminotransferase　(AST)　and　alanine　aminotransferase　(ALT),　and　significantly　inhibited　hepatic　lipid　accumulation　and　steatosis　in　mice　exposed　to　alcohol　intake.　Besides,　the　abnormally　high　levels　of　bile　acids　(BAs)　and　lactate　dehydrogenase　(LDH)　in　the　liver　of　mice　with　alcohol　intake　were　significantly　decreased　by　AAM　intervention,　while　the　hepatic　levels　of　glutathione　(GSH)　and　superoxide　dismutase　(SOD)　were　appreciably　increased.　Compared　with　the　model　group,　AAM　supplementation　significantly　changed　the　composition　of　intestinal　flora　and　up-regulated　the　levels　of　Akkermansia,　Bifidobacterium,　Romboutsia,　Muribaculaceae,　Lachnospiraceae_NK4A136_group,　etc.　Furthermore,　liver　metabolomics　demonstrated　that　AAM　had　a　significant　regulatory　effect　on　the　composition　of　liver　metabolites　in　mice　with　alcohol　intake,　especially　the　metabolites　involved　in　phosphatidylinositol　signaling　system,　ascorbate　and　aldarate　metabolism,　starch　and　sucrose　metabolism,　galactose　metabolism,　alpha-linolenic　acid　metabolism,　glycolysis/gluconeogenesis,　and　biosynthesis　of　unsaturated　fatty　acids.　At　the　gene　level,　AAM　treatment　regulated　the　mRNA　levels　of　lipid　metabolism　and　inflammatory　response　related　genes　in　liver,　including　ACC-1,　FASn,　CPT-1,　CD36,　IFN-gamma,　,　LDLr　and　TNF-alpha.　Conclusively,　these　findings　suggest　that　AAM　has　potential　beneficial　effects　on　alleviating　alcohol-induced　liver　injury　and　is　expected　to　become　a　new　functional　food　ingredient.