Salmonella　is　one　of　the　main　pathogenic　factors　that　cause　foodborne　diseases.　Rapid　and　accurate　detection　of　Salmonella　in　food　is　of　great　importance　to　ensure　food　safety.　Nicking　enzyme-assisted　amplification　(NEAA)　is　one　of　the　promising　isothermal　amplification　methods　finishing　the　in　vitro　amplification　in　∼10　min;　however,　it　suffers　from　nonspecific　amplification　a　lot　(∼70%　products　are　noises).　In　this　paper,　we　introduced　CRISPR/Cas12a　to　specifically　recognize　the　NEAA　amplicons　and　transduce　the　signals　into　turned-on　fluorescent　visual　readouts　(vis-NEAA).　Impressively,　with　this　method,　the　high　efficiency　of　NEAA　has　been　taken　great　advantage　and　the　nonspecific　products　were　successfully　bypassed　at　the　same　time.　In　comparison　to　NEAA-gel　electrophoresis,　vis-NEAA　showed　complete　fidelity　toward　the　presence　of　specific　products,　while　for　real-time　PCR,　it　possesses　equivalent　sensitivity　and　specificity　but　saves　∼80%　of　the　time.　A　level　of　80　CFU/mL　Salmonella　in　spiked　eggs　can　be　detected　on-site　in　∼20　min.　©　2022　American　Chemical　Society