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Abstract:
Salmonella is one of the main pathogenic factors that cause foodborne diseases. Rapid and accurate detection of Salmonella in food is of great importance to ensure food safety. Nicking enzyme-assisted amplification (NEAA) is one of the promising isothermal amplification methods finishing the in vitro amplification in similar to 10 min; however, it suffers from nonspecific amplification a lot (similar to 70% products are noises). In this paper, we introduced CRISPR/Cas12a to specifically recognize the NEAA amplicons and transduce the signals into turned-on fluorescent visual readouts (vis-NEAA). Impressively, with this method, the high efficiency of NEAA has been taken great advantage and the nonspecific products were successfully bypassed at the same time. In comparison to NEAA-gel electrophoresis, vis-NEAA showed complete fidelity toward the presence of specific products, while for real-time PCR, it possesses equivalent sensitivity and specificity but saves similar to 80% of the time. A level of 80 CFU/mL Salmonella in spiked eggs can be detected on-site in similar to 20 min.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
ISSN: 0021-8561
Year: 2022
Issue: 7
Volume: 70
Page: 2401-2409
6 . 1
JCR@2022
5 . 7 0 0
JCR@2023
ESI Discipline: AGRICULTURAL SCIENCES;
ESI HC Threshold:48
JCR Journal Grade:1
CAS Journal Grade:1
Cited Count:
WoS CC Cited Count: 22
SCOPUS Cited Count: 18
ESI Highly Cited Papers on the List: 0 Unfold All
WanFang Cited Count:
Chinese Cited Count:
30 Days PV: 1
Affiliated Colleges: