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author:

Bai, Linlin (Bai, Linlin.) [1] | Wang, Liu (Wang, Liu.) [2] | Huang, Shuqin (Huang, Shuqin.) [3] | Bai, Rong (Bai, Rong.) [4] | Lv, Xucong (Lv, Xucong.) [5] (Scholars:吕旭聪) | Sun, Liping (Sun, Liping.) [6] | Zhang, Fang (Zhang, Fang.) [7] (Scholars:张芳) | Xu, Xiahong (Xu, Xiahong.) [8]

Indexed by:

EI SCIE

Abstract:

Salmonella is one of the main pathogenic factors that cause foodborne diseases. Rapid and accurate detection of Salmonella in food is of great importance to ensure food safety. Nicking enzyme-assisted amplification (NEAA) is one of the promising isothermal amplification methods finishing the in vitro amplification in similar to 10 min; however, it suffers from nonspecific amplification a lot (similar to 70% products are noises). In this paper, we introduced CRISPR/Cas12a to specifically recognize the NEAA amplicons and transduce the signals into turned-on fluorescent visual readouts (vis-NEAA). Impressively, with this method, the high efficiency of NEAA has been taken great advantage and the nonspecific products were successfully bypassed at the same time. In comparison to NEAA-gel electrophoresis, vis-NEAA showed complete fidelity toward the presence of specific products, while for real-time PCR, it possesses equivalent sensitivity and specificity but saves similar to 80% of the time. A level of 80 CFU/mL Salmonella in spiked eggs can be detected on-site in similar to 20 min.

Keyword:

CRISPR/Cas12a nicking enzyme-assisted amplification Salmonella sequence specific visual detection

Community:

  • [ 1 ] [Bai, Linlin]Fuzhou Univ, Coll Biol Sci & Engn, Fuzhou 350108, Peoples R China
  • [ 2 ] [Huang, Shuqin]Fuzhou Univ, Coll Biol Sci & Engn, Fuzhou 350108, Peoples R China
  • [ 3 ] [Bai, Rong]Fuzhou Univ, Coll Biol Sci & Engn, Fuzhou 350108, Peoples R China
  • [ 4 ] [Lv, Xucong]Fuzhou Univ, Coll Biol Sci & Engn, Fuzhou 350108, Peoples R China
  • [ 5 ] [Zhang, Fang]Fuzhou Univ, Coll Biol Sci & Engn, Fuzhou 350108, Peoples R China
  • [ 6 ] [Bai, Linlin]Zhejiang Acad Agr Sci, Inst Agroprod Safety & Nutr, State Key Lab Managing Biot & Chem Threats Qual &, Key Lab Informat Traceabil Agr Prod,Minist Agr &, Hangzhou 310021, Peoples R China
  • [ 7 ] [Wang, Liu]Zhejiang Acad Agr Sci, Inst Agroprod Safety & Nutr, State Key Lab Managing Biot & Chem Threats Qual &, Key Lab Informat Traceabil Agr Prod,Minist Agr &, Hangzhou 310021, Peoples R China
  • [ 8 ] [Sun, Liping]Zhejiang Acad Agr Sci, Inst Agroprod Safety & Nutr, State Key Lab Managing Biot & Chem Threats Qual &, Key Lab Informat Traceabil Agr Prod,Minist Agr &, Hangzhou 310021, Peoples R China
  • [ 9 ] [Xu, Xiahong]Zhejiang Acad Agr Sci, Inst Agroprod Safety & Nutr, State Key Lab Managing Biot & Chem Threats Qual &, Key Lab Informat Traceabil Agr Prod,Minist Agr &, Hangzhou 310021, Peoples R China
  • [ 10 ] [Huang, Shuqin]Fujian Key Lab Inspect & Quarantine Technol Res, Fuzhou 350108, Peoples R China
  • [ 11 ] [Bai, Rong]Fujian Key Lab Inspect & Quarantine Technol Res, Fuzhou 350108, Peoples R China

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Source :

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

ISSN: 0021-8561

Year: 2022

Issue: 7

Volume: 70

Page: 2401-2409

6 . 1

JCR@2022

5 . 7 0 0

JCR@2023

ESI Discipline: AGRICULTURAL SCIENCES;

ESI HC Threshold:48

JCR Journal Grade:1

CAS Journal Grade:1

Cited Count:

WoS CC Cited Count: 22

SCOPUS Cited Count: 18

ESI Highly Cited Papers on the List: 0 Unfold All

WanFang Cited Count:

Chinese Cited Count:

30 Days PV: 1

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