This　study　aimed　to　investigate　the　protective　effects　of　ganoderic　acids　(GA)　from　Ganoderma　lucidum　against　liver　injury　and　intestinal　microbial　disorder　in　mice　with　excessive　alcohol　intake.　Results　showed　GA　supplement　significantly　inhibited　the　abnormal　elevation　of　the　liver　index,　serum　lipid　parameters,　aspartate　aminotransferase　and　alanine　aminotransferase　in　mice　exposed　to　alcohol　intake,　and　also　significantly　protected　the　excessive　lipid　accumulation　and　pathological　changes.　Alcohol-induced　oxidative　stress　in　the　liver　was　significantly　ameliorated　by　GA　intervention　through　reducing　the　levels　of　maleic　dialdehyde　and　lactate　dehydrogenase　and　increasing　the　levels　of　glutathione,　catalase,　superoxide　dismutase　and　alcohol　dehydrogenase.　Intestinal　microbiota　profiling　demonstrated　GA　intervention　modulated　the　composition　of　intestinal　microflora　by　increasing　the　levels　of　Lactobacillus,　Faecalibaculum,　Romboutsia,　Bifidobacterium　and　decreasing　the　Helicobacter　level.　Furthermore,　liver　metabolomic　profiling　suggested　GA　intervention　had　a　remarkable　regulatory　effect　on　liver　metabolism　with　excessive　alcohol　consumption.　Moreover,　GA　intervention　regulated　mRNA　levels　of　alcohol　metabolism,　fatty　lipid　metabolism,　oxidative　stress,　bile　acid　biosynthesis　and　metabolism-related　genes　in　the　liver.　Conclusively,　these　findings　demonstrate　GA　intervention　can　significantly　relieve　alcoholic　liver　injury　and　it　is　hopeful　to　become　a　new　functional　food　ingredient　for　the　prevention　of　alcoholic　liver　injury.