A label-free thrombin photoelectrochemical aptasensor based on structure-switching in G-quadruplexes - Details

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EI Scopus

Abstract：

In　this　work,　an　Au/GaN　photoelectrode　was　prepared　by　sputtering　a　30.0-nm–thick　gold　film　on　an　n-type　gallium　nitride　(GaN)　surface.　Guanine　(G)　was　used　as　a　hole　trap　in　photoinduced　charge　separation　due　to　its　lowest　oxidation　potential　and　because　it　could　be　easily　oxidized　among　the　five　common　nucleobases.　Guanosine-rich　DNA　sequences　can　form　stable　secondary　structures　known　as　G-quadruplexes.　The　efficiency　of　hole　transport　was　significantly　improved　after　G-quadruplexes　formation.　The　thrombin　aptamer　rich　in　guanine　could　be　modified　on　the　electrode　surface　through　Au–S　bonds,　forming　stable　G-quadruplexes　in　the　presence　of　target　thrombin.　Due　to　the　presence　of　the　G-quadruplexes,　the　electron-hole　recombination　rate　of　the　system　decreased　and　the　photocurrent　increased.　Under　optimized　conditions,　high　specificity,　selectivity,　and　linearity　could　be　achieved　in　the　concentration　range　of　0.100–10.0　nm,　and　the　limit　of　detection　was　approximately　20.0　pM.　Thus,　the　detection　results　of　human　serum　thrombin　were　satisfactory.　©　2022　The　Authors

Keyword：

DNA sequences Electrochemistry Enzymes Gallium nitride III-V semiconductors Positive ions

Community：

[ 1 ] [Rong, Xiujun]Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, College of Chemistry, Fuzhou University, Fujian, Fuzhou; 350116, China
[ 2 ] [Zhang, Huilan]Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, College of Chemistry, Fuzhou University, Fujian, Fuzhou; 350116, China
[ 3 ] [Cai, Weicheng]Key Lab of Liaoning IC Technology, School of Biomedical Engineer, Faculty of Electronic Information and Electrical Engineering, Dalian University of Technology, Dalian; 116024, China
[ 4 ] [Luo, Fang]College of Biological Science and Engineering, Fuzhou University, Fujian, Fuzhou; 350116, China
[ 5 ] [Lin, Cuiying]College of Chemistry, Fuzhou University, Fujian, Fuzhou; 350116, China
[ 6 ] [Qiu, Bin]Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, College of Chemistry, Fuzhou University, Fujian, Fuzhou; 350116, China
[ 7 ] [Lin, Zhenyu]Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, College of Chemistry, Fuzhou University, Fujian, Fuzhou; 350116, China
[ 8 ] [Wang, Jian]Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, College of Chemistry, Fuzhou University, Fujian, Fuzhou; 350116, China
[ 9 ] [Jiang, Zhou]College of Chemistry, Fuzhou University, Fujian, Fuzhou; 350116, China
[ 10 ] [Huang, Hui]Key Lab of Liaoning IC Technology, School of Biomedical Engineer, Faculty of Electronic Information and Electrical Engineering, Dalian University of Technology, Dalian; 116024, China