In　this　study,　a　Tara　thangka　(from　a　complete　set　of　21)　dating　to　the　mid-Qing　Dynasty　(1776-1839　CE)　at　Daxingshan　Temple　in　Xi＇an,　Shaanxi,　China　was　analyzed　using　various　techniques,　including　portable　X-ray　fluorescence　spectroscopy　(p-XRF),　Raman　spectroscopy　(RS),　Fourier　transform　infrared　spectroscopy　(FTIR),　optical　microscopy　(OM),　and　mass　spectrometry-based　proteomics.　Through　a　multi-analytical　methodology,　in　situ　non-invasive　testing　was　complemented　with　an　analysis　of　a　limited　number　of　samples.　By　complementary　evidence　derived　from　the　above　analyses,　it　can　be　determined　that　the　thangka　was　painted　on　a　hemp　substrate,　with　common　colorants　such　as　cinnabar,　orpiment,　gold,　azurite,　and　malachite,　and　porcine　glue　as　binding　material.　These　results　provided　important　scientific　data　for　the　production　crafts　of　the　precious　Tara　thangkas,　contributing　to　the　revelation　of　its　value　in　art　history　and　enabling　conservators　to　make　informed　conservation　decisions.