The　aim　of　this　study　is　to　investigate　the　molecular　mechanism　of　Smilax　china　L.　polyphenols　(SCLPs)　in　enhancing　lipid　metabolism　and　stimulating　browning　to　reduce　lipid　accumulation　in　3T3-L1　adipocytes.　SCLP　treatment　obviously　decreased　lipid　content　in　a　dose-dependent　manner　(10-40　mu　g/mL)　in　adipocytes.　SCLP　treatment　cooperated　with　noradrenalin　to　increase　lipolysis.　SCLPs　reduced　the　gene　expressions　of　C/EBP　alpha　and　Ap2　and　enhanced　the　expressions　of　ACO,　CPT,　pHSL/HSL,　ATGL,　and　PKA　in　adipocytes.　Furthermore,　SCLPs　increased　mRNA　and　protein　expressions　of　brown　adipocyte-specific　factors　(UCP-1,　PRDM16,　PGC-1　alpha,　and　PPAR　gamma)　and　mRNA　expressions　of　beige　adipocyte-specific　markers　(CD137,　Tbx1,　and　Tmem26)　in　3T3-L1　adipocytes,　as　well　as　mitochondrial　biogenesis　genes　(Nrf1　and　Tfam).　In　addition,　according　to　the　immunofluorescence　staining,　the　mitochondria　number　was　increased　by　SCLP.　Moreover,　beta　3-AR　or　AMPK　agonist　synergistic　SCLPs　enhanced　the　expressions　of　UCP-1,　PRDM16,　and　PGC-1　alpha.　While　beta　3-AR　or　AMPK　antagonist　significantly　decreased　the　expressions　of　these　brown　adipocyte-specific　factors,　SCLP　treatment　inhibited　the　effect　of　antagonist　to　improve　the　expression　of　UCP-1,　PRDM16,　and　PGC-1　alpha.　These　results　indicated　that　SCLPs　may　regulate　lipid　metabolism　and　stimulate　browning　via　the　beta　3-AR/AMPK　alpha　signaling　pathway.　Thus,　SCLPs　likely　have　potential　therapeutic　effects　on　obesity.