In　this　study,　we　explored　the　effect　of　acid　hydrolysis　on　the　molecular,　structural,　rheological,　thermal,　and　antioxidant　characteristics　of　Qingke　β-glucan.　The　acid　hydrolysis　reduced　the　molecular　weights　of　β-glucans　from　510　to　155　KDa.　The　results　of　the　structural　analysis　by　nuclear　magnetic　resonance　(NMR)　spectroscopy,　X-ray　diffraction,　and　fourier　transforms　infrared　(FTIR)　spectroscopy　indicated　that　acid　hydrolysis　did　not　change　the　primary　functional　groups　of　β-glucans.　The　rheological　behavior　of　β-glucan　without　and　with　acid　hydrolysis　can　be　described　as　pseudoplastic　and　Newtonian,　respectively.　The　DSC　curves　of　the　β-glucans　with　high　molecular　weights　showed　the　highest　transition　temperature.　The　2,　2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic　acid)　(ABTS)　radical　cation　scavenging　activity　and　the　reducing　power　of　soluble　β-glucans　in　Qingke　showed　a　dose-dependent　pattern.　Meanwhile,　the　antioxidant　activities　of　Qingke　β-glucan　of　different　molecular　weights　were　similar.　This　study　demostrated　that　the　acid　hydrolysis　almost　have　no　effect　on　antioxidant　activity　of　Qingke　β-glucans.　Copyright　©　2022　Zhao,　Lin,　Lin,　Wu　and　Chen.