Bisphenol　A　(BPA)　is　a　well-known　estrogenic　endocrine　disrupting　chemical　(EDC)　ubiquitously　present　in　various　environmental　media.　The　present　study　aims　to　identify　the　responsive　genes　in　male　fish　chronically　exposed　to　low　concentrations　of　BPA　at　the　transcription　level.　We　screened　genes　from　a　suppression　subtractive　hybridization　library　constructed　from　male　medaka　(Oryzias　latipes)　livers　after　60-d　exposure　to　10　mu　g/L　BPA　under　the　condition　at　which　changes　of　hepatic　antioxidant　parameters　have　been　previously　reported.　The　identified　genes　were　predicted　to　be　involved　in　multiple　biological　processes　including　antioxidant　physiology,　endocrine　system,　detoxification,　notably　associated　with　the　immune　response　processes.　With　real　time　PCR　analysis,　the　immune-associated　genes　including　hepcidin-like　precursor,　complement　component　and　factors,　MHC　class　I,　alpha-2-macroglobulin　and　novel　immune-type　receptor　6　isoform　were　significantly　up-regulated　in　a　nonmonotonic　dose　response　pattern　in　livers　upon　exposure　to　different　concentrations　of　BPA　(0.1,　1,　10,　100,　1000　mu　g/L).　Our　results　demonstrated　a　negative　impact　on　gene　regulation　in　fish　chronically　exposed　to　relatively　low　and　environmentally　relevant　concentrations　of　BPA,　and　suggested　the　potential　immune　modulatory　effect　of　chronic　EDC　exposure　on　fish.　The　immunotoxicity　of　BPA　and　other　EDCs　should　be　much　concerned　for　the　health　of　human　beings　and　other　vertebrates　exposed　to　it.　(C)　2016　Elsevier　Inc.　All　rights　reserved.