Bisphenol　A　(BPA)　and　nonylphenol　(NP)　are　well　known　endocrine-disrupting　chemicals　(EDCs)　ubiquitous　in　the　aquatic　environment　and　are　an　ecotoxicological　risk　for　the　health　of　aquatic　organisms.　Limited　attention　has　been　given　to　the　immunotoxicity　of　these　chemicals.　The　present　study　revealed　a　concentration-dependent　increase　of　reactive　oxygen　species　content　and　an　induced　expression　of　redox-sensitive　transcription　factors　in　zebrafish　embryos　after　exposure　to　various　concentrations　of　BPA,　NP,　and　BPA/NP　mixture　for　4h　to　168h　postfertilization.　Transcription　of　genes　related　to　the　immune　response,　including　IFN,　IL1,　IL10,　Mx,　TNF,　CC-chemokine,　and　CXCL-clc,　were　significantly　up-regulated　on　exposure　to　EDCs.　A　significant　induction　of　concentrations　of　proinflammatory　mediator,　nitric　oxide,　accompanied　by　an　increase　in　the　activity　of　nitric　oxide　synthase　(NOS)　and　an　upregulation　of　inducible　NOS　gene　expression,　was　detected　in　zebrafish　embryos　on　exposures　to　EDCs.　To　elucidate　the　potential　mechanisms　by　which　BPA　and　NP　activate　the　innate　immune　response,　expression　profiles　of　genes　related　to　the　Toll-like　receptors　(TLRs)　signaling　pathway　were　examined.　Expressions　of　TLR3,　TRIF,　MyD88,　SARM,　IRAK4,　and　TRAF6　were　altered　on　exposure　to　EDCs.　The　authors＇　results　demonstrate　that　exposure　to　BPA　and　NP　significantly　affects　the　expression　of　genes　related　to　immune　response　in　zebrafish　embryos　following　oxidative　stress.　Environ　Toxicol　Chem　2013;32:1793-1799.　(c)　2013　SETAC