Electrical　and　electronic　waste　(E-waste)　has　now　become　the　fastest　growing　solid　waste　around　the　world.　Primitive　recycling　operations　for　E-waste　have　resulted　in　severe　contamination　of　toxic　metals　and　organic　chemicals　in　the　related　areas.　In　this　study,　six　dust　and　soil　samples　collected　from　E-waste　recycling　workshops　and　open-burning　sites　in　Longtang　were　analyzed　to　investigate　their　cytotoxicity　and　genotoxicity　on　L02　cells.　These　six　samples　were:　dust　No.　1　collected　at　the　gate　of　the　workshop;　dust　No.　2　collected　from　air　conditioning　compressor　dismantling　site;　dust　No.　3　collected　from　where　some　motors,　wires,　and　aluminium　products　since　the　1980s　were　dismantled;　soil　No.　1　collected　at　the　circuit　board　acid　washing　site;　soil　No.　2　collected　from　a　wire　open-burning　site;　soil　No.　3　collected　near　a　fiber　open-burning　site.　At　the　same　time,　two　control　soil　samples　were　collected　from　farmlands　approximately　8　km　away　from　the　dismantling　workshops.　The　results　showed　that　all　of　these　samples　could　inhibit　cell　proliferation　and　cause　cell　membrane　lesion,　among　which　dust　No.　3　and　soil　No.　2　had　the　strongest　toxicity.　Moreover,　the　comet　assay　showed　that　the　dust　No.　3　had　the　most　significant　capability　to　cause　DNA　single-strand　beaks　(SSB),　while　the　road　dust　(dust　No.　1)　collected　at　the　gate　of　the　workshop,　a　relatively　farer　site,　showed　the　slightest　capability　to　induce　DNA　SSB.　The　intracellular　reactive　oxygen　species　(ROS)　detection　showed　that　ROS　level　was　elevated　with　the　increase　of　dust　and　soil　samples　concentration.　Dust　No.　3　and　soil　No.　2　had　the　highest　ROS　level,　followed　by　dust　No.　2　and　1,　soil　No.　3　and　1.　All　of　the　above　results　indicated　that　polluted　soil　and　dust　from　the　E-waste　area　had　cytotoxicity　and　genotoxicity　on　L02　cells,　the　mechanism　might　involve　the　increased　ROS　level　and　consequent　DNA　SSB.