Coffee　rings　formed　by　evaporation　of　analyte-containing　droplets　are　widely　observed　in　micropatterning,　bio-arrays,　and　trace　detection.　The　coffee-ring　effect　caused　by　contact　line　pinning　significantly　affects　the　detection　uniformity　and　sensitivity.　Here,　we　propose　a　simple　and　operable　method　to　effectively　suppress　coffee　rings　through　controllable　nanoparticles　aggregation　by　superhydrophobicity-enabled　dynamic　evaporation.　The　gold　nanoparticles　(AuNPs)　deposition　footprint　formed　after　dynamic　evaporation　on　an　integrated　superhydrophobic　surface　was　reduced　by　∼3　orders　of　magnitude　compared　to　that　of　non-interventional　evaporation.　Detailed　experiments,　numerical　simulations,　and　theoretical　studies　have　revealed　that　substrate　wettability,　temperature　and　droplet　motion　behaviors　play　significant　roles　in　suppressing　coffee-ring　effect.　More　critically,　based　on　the　force　mechanism　of　AuNPs　at　the　interface/contact　line,　universal　mathematical　models　and　regime　maps　were　established　to　classify　the　different　deposition　modes　for　AuNPs　under　different　evaporation　conditions　by　introducing　dimensionless　parameter　G,　revealing　the　enrichment　mechanism　of　AuNPs　in　droplets　under　superhydrophobicity-enabled　dynamic　evaporation.　The　accuracy　of　the　theoretical　model　and　enrichment　mechanism　was　demonstrated　through　the　single-molecule　detection　of　rhodamine　6G　with　excellent　sensitivity　(10–17　M,　enhancement　factor　∼1013)　and　perfect　uniformity　(relative　standard　deviation　∼5.57　%),　which　provides　a　valuable　guide　for　research　and　applications　of　nanoparticle　aggregation.　©　2024　Elsevier　Inc.