Background　White-rot　fungi　are　known　to　naturally　produce　high　quantities　of　laccase,　which　exhibit　commendable　stability　and　catalytic　efficiency.　However,　their　laccase　production　does　not　meet　the　demands　for　industrial-scale　applications.　To　address　this　limitation,　it　is　crucial　to　optimize　the　conditions　for　laccase　production.　However,　the　regulatory　mechanisms　underlying　different　conditions　remain　unclear.　This　knowledge　gap　hinders　the　cost-effective　application　of　laccases.　Results　In　this　study,　we　utilized　transcriptomic　and　metabolomic　data　to　investigate　a　promising　laccase　producer,　Cerrena　unicolor　87613,　cultivated　with　fructose　as　the　carbon　source.　Our　comprehensive　analysis　of　differentially　expressed　genes　(DEGs)　and　differentially　abundant　metabolites　(DAMs)　aimed　to　identify　changes　in　cellular　processes　that　could　affect　laccase　production.　As　a　result,　we　discovered　a　complex　metabolic　network　primarily　involving　carbon　metabolism　and　amino　acid　metabolism,　which　exhibited　contrasting　changes　between　transcription　and　metabolic　patterns.　Within　this　network,　we　identified　five　biomarkers,　including　succinate,　serine,　methionine,　glutamate　and　reduced　glutathione,　that　played　crucial　roles　in　co-determining　laccase　production　levels.　Conclusions　Our　study　proposed　a　complex　metabolic　network　and　identified　key　biomarkers　that　determine　the　production　level　of　laccase　in　the　commercially　promising　Cerrena　unicolor　87613.　These　findings　not　only　shed　light　on　the　regulatory　mechanisms　of　carbon　sources　in　laccase　production,　but　also　provide　a　theoretical　foundation　for　enhancing　laccase　production　through　strategic　reprogramming　of　metabolic　pathways,　especially　related　to　the　citrate　cycle　and　specific　amino　acid　metabolism.