• Complex
  • Title
  • Keyword
  • Abstract
  • Scholars
  • Journal
  • ISSN
  • Conference
成果搜索

author:

Chen, J. (Chen, J..) [1] | Zhang, X. (Zhang, X..) [2] | Lu, H. (Lu, H..) [3] | Wu, Y. (Wu, Y..) [4] | Xu, Y. (Xu, Y..) [5] | Guo, L. (Guo, L..) [6]

Indexed by:

Scopus

Abstract:

Monitoring mitochondrial pH and differentiating live and dead cells are crucial for diagnosing cell status. However, most fluorescent probes suffer from limitations such as high cytotoxicity, photobleaching, unreliability, and an inability to differentiate cell death caused by different inducers. Herein, a ratiometric fluorescent nanoprobe was developed by assembling pH-sensitive hydroxyl- and phenyl-co-modified carbon nitride (HPCN) with pH-insensitive Rhodamine B (RB). HPCN was prepared via thermal condensation of phenylguanidine carbonate using NaOH as the melt. The hydroxyl group modification endowed HPCN with improved water solubility and pH-sensitive characteristics, while the phenyl group modification facilitated mitochondrial targeting and DNA staining via hydrophobic interactions. Based on the fluorescence resonance energy transfer (FRET) from HPCN to RB, the nanoprobe exhibited a linear response in the relative fluorescence intensities at 500 nm and 584 nm over a pH range of 4.5–8.5. Benefiting from its low cytotoxicity, excellent reversibility, and outstanding photostability, the nanoprobe was capable of monitoring mitochondrial pH changes in live cells and differentiating live and dead cells, apoptosis and necrosis, and necrosis induced by different agents, regardless of cell type. This work provides a reliable method for diagnosing cell status and cell death induced by various inducers. © 2025 Elsevier B.V.

Keyword:

Carbon nitride Cell differentiation Mitochondrial pH Nanoprobe Ratiometric fluorescence

Community:

  • [ 1 ] [Chen J.]Ministry of Education Key Laboratory for Analytical Science of Food Safety and Biology, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, College of Chemistry, Fuzhou University, Fuzhou, 350116, China
  • [ 2 ] [Zhang X.]Ministry of Education Key Laboratory for Analytical Science of Food Safety and Biology, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, College of Chemistry, Fuzhou University, Fuzhou, 350116, China
  • [ 3 ] [Lu H.]Ministry of Education Key Laboratory for Analytical Science of Food Safety and Biology, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, College of Chemistry, Fuzhou University, Fuzhou, 350116, China
  • [ 4 ] [Wu Y.]Ministry of Education Key Laboratory for Analytical Science of Food Safety and Biology, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, College of Chemistry, Fuzhou University, Fuzhou, 350116, China
  • [ 5 ] [Xu Y.]Department of Otorhinolaryngology, First Affiliated Hospital of Fujian Medical University, Fuzhou, 350001, China
  • [ 6 ] [Guo L.]Ministry of Education Key Laboratory for Analytical Science of Food Safety and Biology, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, College of Chemistry, Fuzhou University, Fuzhou, 350116, China

Reprint 's Address:

Email:

Show more details

Related Keywords:

Source :

Talanta

ISSN: 0039-9140

Year: 2025

Volume: 291

5 . 6 0 0

JCR@2023

Cited Count:

WoS CC Cited Count:

SCOPUS Cited Count:

ESI Highly Cited Papers on the List: 0 Unfold All

WanFang Cited Count:

Chinese Cited Count:

30 Days PV: 1

Affiliated Colleges:

Online/Total:142/10048735
Address:FZU Library(No.2 Xuyuan Road, Fuzhou, Fujian, PRC Post Code:350116) Contact Us:0591-22865326
Copyright:FZU Library Technical Support:Beijing Aegean Software Co., Ltd. 闽ICP备05005463号-1