In　this　work,　novel　Cu2V4O11　nanorods　(CVNRs)　were　meticulously　synthesized　employing　an　exquisite　one-pot　hydrothermal　technique,　thereby　endowed　with　an　inherent　peroxidase-like　prowess　capable　of　catalyzing　the　oxidation　of　3,3′,5,5′-tetramethylbenzidine　(TMB)　to　produce　a　blue　color　reaction　in　the　presence　of　H2O2.　Kinetic　examinations　revealed　that　CVNRs　exhibited　a　3.36-fold　increased　affinity　for　TMB　in　comparison　to　the　natural　enzyme　horseradish　peroxidase,　and　the　catalytic　response　derived　from　CVNRs　adhered　to　a　ping-pong　mechanism.　Free　radical　trapping　experiments　corroborated　the　pivotal　role　of　·O2−　radicals　in　establishing　the　dominant　oxidant　for　TMB　oxidation.　Hence,　a　sensitive　visual　assessment　platform,　grounded　in　CVNRs,　was　engineered　for　the　detection　of　H2O2　and　glucose.　The　limit　of　detection　for　H2O2　and　glucose　were　0.054　μM　and　2.24　μM,　respectively,　and　the　detection　ranges　were　1–50　μM　and　10–75　μM,　respectively.　Most　notably,　the　visual　platform　harbored　exceptional　stability,　selectivity,　and　anti-interference　capabilities.　This　research　not　only　bestows　upon　CVNRs　an　extraordinary　capability　to　discern　H2O2　and　glucose　within　practical　samples,　but　also　expands　the　realm　of　application　for　CVNRs　in　the　domains　of　environmental　preservation,　forensic　science　and　biomedical　diagnosis.　©　2025　Elsevier　Masson　SAS