Pseudomonas　aeruginosa,　recognized　for　its　biofilm　production　and　secretion　of　virulence　factors,　posing　a　severe　threat　in　areas　such　as　clinical　infections,　food　contamination,　and　marine　biofouling.　To　address　this,　a　new　type　of　zinc-binding　peptide　(CSSP-Zn)　was　prepared　from　crimson　snapper　scales　peptides　(CSSP)　and　goslarite,　and　its　antibacterial　and　anti-quorum-sensing　activities　toward　P.　aeruginosa　PAO1　were　exploited.　Results　indicated　that　CSSP-Zn　induced　planktonic　strain　PAO1　membrane　injury　via　inhibiting　expression　levels　of　cell　integrity　genes,　targeting　microbial-specific　membrane　constituents,　disrupting　proton　motive　force,　and　causing　metabolic　disturbances.　Meanwhile,　CSSP-Zn　decreased　virulence　factors　pyocyanin,　protease　and　rhamnolipid　secretion,　while　considerably　inhibiting　quorum　sensing-related　genes　(las,　pqs　and　rhl)　expression　and　decreasing　bacterial　abundance　and　pathogenicity　in　fish　models.　Moreover,　CSSP-Zn　not　only　effectively　hindered　biofilm　formation　but　also　disassembled　preformed　ones,　thus　disrupting　biofilm　topology.　Taken　together,　utilizing　food　byproducts　to　obtain　CSSP-Zn　could　help　recycle　food　resources　and　provide　insight　into　controlling　planktonic　and　biofilm　strain　PAO1　contamination.