Dietary　peptide　has　been　of　great　interest　because　of　its　perspective　in　nutrition　and　health　of　human　body.　The　aim　of　this　study　was　to　develop　a　dietary　nutritional　supplement　exerting　both　antioxidant　and　anti-aging　effects.　Peptide,　named　as　ERJ-CP,　was　prepared　by　mixing　enzyme-treated　royal　jelly　(ERJ)　with　collagen　peptide　(CP),　showing　stronger　antioxidant　activity　in　vitro.　Drosophila　was　used　as　model　animal　to　investigate　anti-aging　effect　of　ERJ-CP　in　vivo.　ERJ-CP　significantly　prolonged　the　average　life　span　of　Drosophila　treated　with　H2O2　and　paraquat,　reducing　malondialdehyde　(MDA)　and　protein　carbonyl　(PCO)　levels　in　Drosophila.　In　addition,　3　mg/mL　of　ERJ-CP　could　prolong　the　lifespan　of　natural　aging　Drosophila　by　11.16%.　ERJ-CP　could　upregulate　the　levels　of　total　superoxide　dismutase　(T-SOD),　glutathione　peroxidase　(GSH-Px),　catalase　(CAT)　and　down-regulate　the　contents　of　MDA　and　PCO.　Moreover,　the　intake　of　ERJ-CP　increased　the　food　consumption,　weight　gain　and　exercise　capacity　of　Drosophila.　The　results　showed　that　ERJ-CP　played　a　protective　role　in　both　antioxidant　and　anti-aging　effects　on　Drosophila,　and　the　anti-aging　effect　may　be　achieved　by　alleviating　oxidative　damage.　It　suggests　that　ERJ-CP　could　be　developed　as　a　health-promoting　ingredient　with　antioxidant　and　anti-aging　effects　for　human　body.