Adenosine　5＇-triphosphate　(ATP),　as　a　＂molecular　currency＂　for　intracellular　energy　transfer,　not　only　participates　in　various　physiological　activities,　but　also　involves　in　various　pathological　processes　of　living　cells.　Therefore,　the　establishment　of　a　simple,　rapid,　sensitive　and　accurate　ATP　analysis　method　is　of　great　significance　for　the　study　of　its　physiological　functions.　In　this　work,　we　rationally　design　a　near-infrared　phenylboronic　acid　group-functionalized　dicyanovinyl　squaraine　(SQ-　PBA3),　with　which　a　supramolecular　assembly　was　constructed　for　fluorescent　imaging　of　ATP.　In　PBS　buffer,　SQ-PBA3　changes　self-assembly　when　adding　cetyltrimethyl　ammonium　bromide　(CTAB)　deriving　fluorescence　response　of　SQ-PBA3　to　ATP　with　the　maximum　emission　at　700　nm,　due　to　the　specificity　of　phenylboronic　acid　to　diols　and　the　multiple　electrostatic　interactions　between　SQ　PBA3,　ATP　and　CTAB　molecules.　Meanwhile,　the　probe　was　successfully　applied　to　monitor　intracellular　ATP　level　in　MCF-7　cells　with　high　sensitivity　and　selectivity.