Via　the　facile　ring-opening　reaction　of　epoxy　groups　with　quinine,　a　novel　polymer　monolith　with　quaternary　ammonium　for　reversed-phase/strong　anion-exchange　mixed-mode　has　been　fabricated　for　pressurized　capillary　electrochromatography　(pCEC).　Optimization　on　the　preparation　of　quinine-modified　monoliths　has　been　investigated,　and　characteristics　including　morphology,　permeability,　mechanical　stability,　reproducibility,　and　column　performance　have　been　also　studied.　Active　quaternary　ammonium　groups　were　conveniently　produced　to　generate　cationic　action　sites　and　stable　anodic　electroosmotic　flow.　Multiple　interactions　including　reversed-phase,　strong　anion-exchange,　electrostatic　repulsion　and　-　stacking　interactions　were　obtained.　Satisfactory　separation　capability　of　various　analytes　such　as　alkylbenzenes,　polycyclic　aromatic　hydrocarbons,　benzoic　acid　and　its　homologs,　and　(2)-receptor　excitants　has　been　achieved.　Applied　to　the　real　sample,　the　good　resolution　of　three　alkaloids　in　Corydalis　yanhusuo　were　achieved　by　pCEC　with　the　quinine-modified　monolith.　The　results　light　a　potential　access　to　facilely　fabricating　quaternary　ammonium-functionalized　polymer　monolith　with　multiple　interactions　for　efficient　electrochromatography　profiling　of　various　compounds.