Impaired　endothelial　functions　are　closely　associated　with　many　chronic　vascular-related　diseases.　Maslinic　acid　(MA),　a　natural　pentacyclic　triterpene　compound,　has　received　more　and　more　attentions　in　recent　years　due　to　a　variety　of　bioactivities.　In　the　present　study,　we　investigated　the　effect　of　MA　on　impaired　endothelial　functions　in　human　aortic　endothelial　cells　(HAEC)　induced　by　high　glucose　treatment　and　discussed　its　possible　mechanism.　We　showed　that　MA　decreased　the　ROS　level,　inhibited　the　inflammatory　cytokines　expression,　facilitated　insulin-mediated　IRS-1/PI3K/Akt/eNOS　signaling　and　suppressed　the　cellular　apoptosis　ratio　induced　by　high　glucose.　The　molecular　docking　results　showed　that　MA　may　regulate　multiple　targets　to　improve　the　endothelial　dysfunction　in　HAECs　exposed　to　high　glucose.　These　results　revealed　potential　application　of　MA　in　improving　endothelia　dysfunction.