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author:

Li, Feng (Li, Feng.) [1] (Scholars:李锋) | Zhao, Hui (Zhao, Hui.) [2] | Wang, Zheng-Yong (Wang, Zheng-Yong.) [3] | Wu, Zai-Sheng (Wu, Zai-Sheng.) [4] (Scholars:吴再生) | Yang, Zhe (Yang, Zhe.) [5] | Li, Cong-Cong (Li, Cong-Cong.) [6] | Xu, Huo (Xu, Huo.) [7] | Lyu, Jian-Xin (Lyu, Jian-Xin.) [8] | Shen, Zhi-Fa (Shen, Zhi-Fa.) [9]

Indexed by:

EI Scopus SCIE

Abstract:

The detection of biomarkers is of crucial importance in reducing the morbidity and mortality of complex diseases. Thus, there is a great desire to develop highly efficient and simple sensing methods to fulfill the different diagnostic and therapeutic purposes. Herein, using tumor suppressor p53 gene as model target DNA, we developed a novel palindromic fragment-incorporated molecular beacon (P-MB) that can perform multiple functions, including recognition element, signal reporter, polymerization template and primer. Upon specific hybridization with target DNA, P-MBs can interact with each other and are extended by polymerase without any additional probes. As a result, hybridized targets are peeled off from P-MBs and initiate the next round of reactions, leading to the unique strand displacement amplification (SDA). The newly-proposed enzymatic amplification displays the detection limit as low as 100 pM and excellent selectivity in distinguishing single-base mutation with the linear response range from 100 pM to 75 nM. This is the simplest SDA sensing system so far because of only involving one type of DNA probe. This impressive sensing paradigm is expected to provide new insight into developing new-type of DNA probes that hold tremendous potential with important applications in molecular biology research and clinical diagnosis.

Keyword:

p53 gene Palindromic fragment-incorporated molecular beacon (P-MB) Strand displacement amplification (SDA)

Community:

  • [ 1 ] [Li, Feng]Wenzhou Med Univ, Minist Educ China, Sch Lab Med & Life Sci,Key Lab Lab Med, Inst Funct Nucle Acids & Personalized Canc Theran, Wenzhou 325035, Peoples R China
  • [ 2 ] [Zhao, Hui]Wenzhou Med Univ, Minist Educ China, Sch Lab Med & Life Sci,Key Lab Lab Med, Inst Funct Nucle Acids & Personalized Canc Theran, Wenzhou 325035, Peoples R China
  • [ 3 ] [Wang, Zheng-Yong]Wenzhou Med Univ, Minist Educ China, Sch Lab Med & Life Sci,Key Lab Lab Med, Inst Funct Nucle Acids & Personalized Canc Theran, Wenzhou 325035, Peoples R China
  • [ 4 ] [Lyu, Jian-Xin]Wenzhou Med Univ, Minist Educ China, Sch Lab Med & Life Sci,Key Lab Lab Med, Inst Funct Nucle Acids & Personalized Canc Theran, Wenzhou 325035, Peoples R China
  • [ 5 ] [Shen, Zhi-Fa]Wenzhou Med Univ, Minist Educ China, Sch Lab Med & Life Sci,Key Lab Lab Med, Inst Funct Nucle Acids & Personalized Canc Theran, Wenzhou 325035, Peoples R China
  • [ 6 ] [Li, Feng]Fuzhou Univ, Canc Metastasis Alert & Prevent Ctr, Coll Chem,Pharmaceut Photocatalysis State Key Lab, Fujian Prov Key Lab Canc Metastasis Chemoprevent, Fuzhou 350002, Peoples R China
  • [ 7 ] [Zhao, Hui]Fuzhou Univ, Canc Metastasis Alert & Prevent Ctr, Coll Chem,Pharmaceut Photocatalysis State Key Lab, Fujian Prov Key Lab Canc Metastasis Chemoprevent, Fuzhou 350002, Peoples R China
  • [ 8 ] [Wu, Zai-Sheng]Fuzhou Univ, Canc Metastasis Alert & Prevent Ctr, Coll Chem,Pharmaceut Photocatalysis State Key Lab, Fujian Prov Key Lab Canc Metastasis Chemoprevent, Fuzhou 350002, Peoples R China
  • [ 9 ] [Yang, Zhe]Fuzhou Univ, Canc Metastasis Alert & Prevent Ctr, Coll Chem,Pharmaceut Photocatalysis State Key Lab, Fujian Prov Key Lab Canc Metastasis Chemoprevent, Fuzhou 350002, Peoples R China
  • [ 10 ] [Li, Cong-Cong]Fuzhou Univ, Canc Metastasis Alert & Prevent Ctr, Coll Chem,Pharmaceut Photocatalysis State Key Lab, Fujian Prov Key Lab Canc Metastasis Chemoprevent, Fuzhou 350002, Peoples R China
  • [ 11 ] [Xu, Huo]Fuzhou Univ, Canc Metastasis Alert & Prevent Ctr, Coll Chem,Pharmaceut Photocatalysis State Key Lab, Fujian Prov Key Lab Canc Metastasis Chemoprevent, Fuzhou 350002, Peoples R China

Reprint 's Address:

  • 吴再生

    [Lyu, Jian-Xin]Wenzhou Med Univ, Minist Educ China, Sch Lab Med & Life Sci,Key Lab Lab Med, Inst Funct Nucle Acids & Personalized Canc Theran, Wenzhou 325035, Peoples R China;;[Shen, Zhi-Fa]Wenzhou Med Univ, Minist Educ China, Sch Lab Med & Life Sci,Key Lab Lab Med, Inst Funct Nucle Acids & Personalized Canc Theran, Wenzhou 325035, Peoples R China;;[Wu, Zai-Sheng]Fuzhou Univ, Canc Metastasis Alert & Prevent Ctr, Coll Chem,Pharmaceut Photocatalysis State Key Lab, Fujian Prov Key Lab Canc Metastasis Chemoprevent, Fuzhou 350002, Peoples R China

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Source :

BIOSENSORS & BIOELECTRONICS

ISSN: 0956-5663

Year: 2017

Volume: 91

Page: 692-698

8 . 1 7 3

JCR@2017

1 0 . 7 0 0

JCR@2023

ESI Discipline: CHEMISTRY;

ESI HC Threshold:226

JCR Journal Grade:1

CAS Journal Grade:1

Cited Count:

WoS CC Cited Count: 28

SCOPUS Cited Count: 27

ESI Highly Cited Papers on the List: 0 Unfold All

WanFang Cited Count:

Chinese Cited Count:

30 Days PV: 1

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