Citrinin　(CIT)-protein　synthetic　antigen　was　prepared　by　conjugating　CIT　with　keyhole　limpet　hemocyanin　by　1,4-butanediol　diglycidyl　ether.　The　ultraviolet　and　infrared　spectrometry　analyses　indicated　that　CIT　was　conjugated　with　the　carrier　protein　successfully.　By　fusion　and　cloning,　a　hybridoma　cell　line　K2-F3　which　stably　secreted　the　highly　specific　monoclonal　antibody　(McAb)　against　CIT　was　obtained.　The　indirect　competitive　ELISA　showed　that　the　IC50　value　was　0.3　ng/mL　and　the　limit　of　detection,　0.01　ng/mL.　Cross-reactivity　was　less　than　0.01%　with　four　mycotoxins,　aflatoxin　B-1,　ochratoxin　A,　zearalenone,　deoxynilvalenol　and　three　pigments,　rubropunctatine,　rubropunctamine,　monascin.　The　mean　recovery　of　citrinin　from　artificially　contaminated　red　yeast　rice　was　from　89　to　92%,　with　CVs　from　6.8　to　9.0%.　The　ELISA　developed　in　this　study　can　accurately　determine　CIT　in　artificially　contaminated　red　yeast　rice.