A　novel　polyoxovanadium　borate　Mn-2[V12B16O52(OH)(6)](en)(2)(H3O)(6)(H2O)(5)　1　(en=ethylenediamine)　was　hydrothermally　synthesized　and　characterized.　by　IR,　two-dimensional　infrared　(2D　IR)　correlation　spectroscopy　with　magnetic　and　thermal　perturbation,　and　single　crystal　X-ray　diffraction.　In　1,　it　is　interesting　that　each　[V12B16O52(OH)(6)](14-)　units　is　connected　by　four　[Mn(H2O)(2)](2+)　to　generate　a　2D　layer　on　the　ab　plane.　Along　c　axis,　these　layers　are　further　linked　by　H-bond　to　form　a　3D　framework.　The　response　of　the　stretching　vibrations　of　B-O,　V-O-V　and　Mn-O-B　was　detected　in　the　2D　IR　correlation　spectra　with　magnetic　perturbation.　In　addition,　the　response　of　the　stretching　vibrations　of　B-OH,　B-O,　V-O-V　and　Mn-O-B　was　detected　in　the　2D　IR　correlation　spectra　with　thermal　perturbation.