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author:

Wang, ZongWen (Wang, ZongWen.) [1] | Wang, WenJian (Wang, WenJian.) [2] | Wang, Wei (Wang, Wei.) [3] (Scholars:王伟) | Xu, LiangJun (Xu, LiangJun.) [4] (Scholars:徐两军) | Chen, GuoNan (Chen, GuoNan.) [5] (Scholars:陈国南) | Fu, FengFu (Fu, FengFu.) [6] (Scholars:付凤富)

Indexed by:

EI Scopus SCIE

Abstract:

A simple, reliable and reproducible method for the separation and determination of five beta-casomorphins (beta-CMs, namely TPGN, PGPI, TPGI, TPGP and TPPG) based on glass microfluidic chip electrophoresis and laser-induced fluorescence detection is first described in here. The microfluidic chip electrophoresis and laser-induced fluorescence detection system consisted of a home-made glass "double-T'' microchip and a simple LIF detector with excitation and emission wavelengths of 473 and 525 nm, respectively. Fluorescein isothiocyanate (FITC) was used as the precolumn derivatization reagent to label fluorophore on five beta-CMs, and the optimum conditions of FITC-derivatization reaction and MCE separation were investigated in detail. Under optimum conditions, five beta-CMs were completely separated and detected within 30 min with a detection limit of 18.7-75.1 nmol/L and an RSD (n = 55) of 3.0-5.9%, respectively. The proposed method has been successfully used to detect beta-CMs in real cheese sample with a recovery of 89-109%, suggesting that our method is sensitive and reliable. These features, as well as its low cost, operation convenience, stability and reusability, make it a promising alternative to beta-CMs detection methods.

Keyword:

beta-Casomorphins Laser-induced fluorescence Microfluidic chip Peptide

Community:

  • [ 1 ] [Wang, ZongWen]Fuzhou Univ, Fujian Prov Key Lab Anal & Detect Food Safety, Key Lab Anal & Detect Food Safety, Minist Educ,Dept Chem, Fuzhou 350108, Fujian, Peoples R China
  • [ 2 ] [Wang, WenJian]Fuzhou Univ, Fujian Prov Key Lab Anal & Detect Food Safety, Key Lab Anal & Detect Food Safety, Minist Educ,Dept Chem, Fuzhou 350108, Fujian, Peoples R China
  • [ 3 ] [Wang, Wei]Fuzhou Univ, Fujian Prov Key Lab Anal & Detect Food Safety, Key Lab Anal & Detect Food Safety, Minist Educ,Dept Chem, Fuzhou 350108, Fujian, Peoples R China
  • [ 4 ] [Xu, LiangJun]Fuzhou Univ, Fujian Prov Key Lab Anal & Detect Food Safety, Key Lab Anal & Detect Food Safety, Minist Educ,Dept Chem, Fuzhou 350108, Fujian, Peoples R China
  • [ 5 ] [Chen, GuoNan]Fuzhou Univ, Fujian Prov Key Lab Anal & Detect Food Safety, Key Lab Anal & Detect Food Safety, Minist Educ,Dept Chem, Fuzhou 350108, Fujian, Peoples R China
  • [ 6 ] [Fu, FengFu]Fuzhou Univ, Fujian Prov Key Lab Anal & Detect Food Safety, Key Lab Anal & Detect Food Safety, Minist Educ,Dept Chem, Fuzhou 350108, Fujian, Peoples R China

Reprint 's Address:

  • 付凤富

    [Fu, FengFu]Fuzhou Univ, Fujian Prov Key Lab Anal & Detect Food Safety, Key Lab Anal & Detect Food Safety, Minist Educ,Dept Chem, Fuzhou 350108, Fujian, Peoples R China

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Source :

JOURNAL OF SEPARATION SCIENCE

ISSN: 1615-9306

Year: 2011

Issue: 2

Volume: 34

Page: 196-201

2 . 7 3 3

JCR@2011

2 . 8 0 0

JCR@2023

ESI Discipline: CHEMISTRY;

JCR Journal Grade:2

CAS Journal Grade:3

Cited Count:

WoS CC Cited Count: 16

SCOPUS Cited Count: 18

ESI Highly Cited Papers on the List: 0 Unfold All

WanFang Cited Count:

Chinese Cited Count:

30 Days PV: 1

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