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Abstract:
A simple, reliable and reproducible method for the separation and determination of five beta-casomorphins (beta-CMs, namely TPGN, PGPI, TPGI, TPGP and TPPG) based on glass microfluidic chip electrophoresis and laser-induced fluorescence detection is first described in here. The microfluidic chip electrophoresis and laser-induced fluorescence detection system consisted of a home-made glass "double-T'' microchip and a simple LIF detector with excitation and emission wavelengths of 473 and 525 nm, respectively. Fluorescein isothiocyanate (FITC) was used as the precolumn derivatization reagent to label fluorophore on five beta-CMs, and the optimum conditions of FITC-derivatization reaction and MCE separation were investigated in detail. Under optimum conditions, five beta-CMs were completely separated and detected within 30 min with a detection limit of 18.7-75.1 nmol/L and an RSD (n = 55) of 3.0-5.9%, respectively. The proposed method has been successfully used to detect beta-CMs in real cheese sample with a recovery of 89-109%, suggesting that our method is sensitive and reliable. These features, as well as its low cost, operation convenience, stability and reusability, make it a promising alternative to beta-CMs detection methods.
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JOURNAL OF SEPARATION SCIENCE
ISSN: 1615-9306
Year: 2011
Issue: 2
Volume: 34
Page: 196-201
2 . 7 3 3
JCR@2011
2 . 8 0 0
JCR@2023
ESI Discipline: CHEMISTRY;
JCR Journal Grade:2
CAS Journal Grade:3
Cited Count:
WoS CC Cited Count: 16
SCOPUS Cited Count: 18
ESI Highly Cited Papers on the List: 0 Unfold All
WanFang Cited Count:
Chinese Cited Count:
30 Days PV: 1
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