A　new　electrochemiluminescence　(ECL)　sensing　interface　was　established　based　on　the　zinc　oxide　nanorod　in　this　paper.　Firstly,　the　zinc　oxide　(ZnO)　nanorod　was　prepared　on　an　indium　tin　oxide　(ITO)　electrode　surface　by　the　method　of　constant　current　cathodic　electrodeposition,　on　which　the　Nation　film　was　then　modified,　and　finally　ruthenium(II)　tris(bipyridine)　(Ru(bpy)(3)(2+))　was　immobilized　at　the　ZnO　nanorod/Nafion　composite-modified　electrode.　The　sensing　interface　shows　well　ECL　behaviors　and　perfect　stability　after　being　constant　temperature　treatment　at　80　degrees　C.　The　composite　electrode　was　characterized　by　EIS,　SEM　and　XRD.　The　results　showed　that　the　good　stability　maybe　related　to　the　water　content　of　Nafion　film.