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A new fluorimetric method has been developed for the determination of deoxyribonucleic acid (DNA) with toluidine blue (TB) as a fluorescence probe. It is based on the fluorescence quenching of toluidine blue in the presence of DNA. In Tris-HCl buffer solution (pH 8.5), the calibration graph was linear over the range 0.10-6.00 mu g(.)mL(-1) for ctDNA, and the detection limit was 27 mu g(.)mL(-1). The result of the determination of DNA of camphor tree＇s leaf by this method was satisfactory.
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SPECTROSCOPY AND SPECTRAL ANALYSIS
ISSN： 1000-0593
CN： 11-2200/O4
Year： 2006
Issue： 1
Volume： 26
Page： 121-124
0 . 6 6 6
JCR@2006
0 . 7 0 0
JCR@2023
ESI Discipline： CHEMISTRY;
JCR Journal Grade：4
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SCOPUS Cited Count：
ESI Highly Cited Papers on the List： 0 Unfold All
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30 Days PV： 0
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