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Abstract:
Capillary electrophoresis (CE) of erythrocytes from different sources under various conditions is reported in this paper. It was found that erythrocyte samples from sheep, duck, and human showed characteristic and reproducible elution peaks, and that the retention times of A-, B-, AB-, and O-type erythrocytes from human blood were distinctively different; even subtle differences, among individuals with the same blood type could be detected by CE. A strictly linear correlation was obtained between the peak area and the amount of human erythrocyte over a range of 4.8 x 10(2)-1.9 x 10(4) cells (r = 0.999), indicating that CE could be used for rapid and accurate quantification of erythrocytes. Using this CE protocol, the decrease of the surface electrical charge of erythrocyte during storage was confirmed. Therefore, this work demonstrated that CE could be a useful alternative for characterizing and quantifying erythrocytes or other cells. (C) 2003 Elsevier Science (USA). All rights reserved.
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ANALYTICAL BIOCHEMISTRY
ISSN: 0003-2697
Year: 2003
Issue: 2
Volume: 314
Page: 194-198
2 . 1 7 4
JCR@2003
2 . 6 0 0
JCR@2023
ESI Discipline: BIOLOGY & BIOCHEMISTRY;
JCR Journal Grade:2
Cited Count:
WoS CC Cited Count: 17
SCOPUS Cited Count: 19
ESI Highly Cited Papers on the List: 0 Unfold All
WanFang Cited Count:
Chinese Cited Count:
30 Days PV: 0
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