Vitreoscilla　hemoglobin　gene　was　expressed　in　S.　aureofaciens　through　promoter　of　tetracycline　resistance　gene.　Characteristics　of　S.　aureofaciens　growth　and　metabolism　were　studied　in　1　m3　fermenter.　In　high　dissolved　oxygen　concentrations,　expression　of　hemoglobin　gene　had　little　effect　on　growth　and　metabolism　of　S.　aureofaciens,　and　there　were　no　obvious　differences　between　engineering　strain　and　control.　The　chlortetracycline　of　engineering　strain　was　22905　u/mL　and　of　control　was　22896　u/mL　respectively.　Under　conditions　of　low　dissolved　oxygen,　expression　of　hemoglobin　enhanced　growth,　maintenance　of　energetic　mycelium　configuration　and　chlortetracycline　yield　of　S.　aureofaciens:　the　mycelium　concentrations　of　engineering　strain　were　more　about　5%-10%　and　yield　was　more　11.4%　than　control.