Hydrogen　sulfide　(H2S)　has　emerged　as　an　important　indicator　in　the　spoilage　process　of　meat.　In　this　study,　a　mimetic　enzyme　based　on　Cu2+-modified　boron　nitride　nanosheets-supported　gold　nanoparticles　(AuNPs/Cu2+-　BNNS)　was　synthesized,　which　can　be　used　to　catalyze　the　oxidation　of　3,3　&　PRIME;,5,5　&　PRIME;-tetramethylbenzidine　(TMB).　The　H2S　gas　can　inhibit　the　activity　of　AuNPs/Cu2+-BNNS　toward　catalytic　oxidation　of　TMB.　Meanwhile,　the　usage　of　headspace　method　could　avoid　most　interferences　in　the　rotten　sample.　Various　concentrations　of　TMB+　could　change　the　aspect　ratio　of　the　gold　nanoroads　(AuNRs),　which　results　in　vivid　color　changing　and　UV-vis　spectra　shifting.　The　sensor　had　a　good　linear　relationship　with　H2S　concentration　ranging　from　10.0　to　90.0　mu　mol/L,　and　the　detection　limit　is　7.8　mu　mol/L.　The　AuNPs/Cu2+-BNNS　sensors　were　successfully　applied　to　detect　H2S　produced　by　meat　spoilage　with　satisfying　results.