Rapid　and　accurate　detection　of　Salmonella　is　extremely　important　to　ensure　food　safety.　Combining　nucleic　acid　amplification　with　CRISPR/Cas12a　can　realize　sensitive　and　specific　detection　of　foodborne　pathogens.　However,　present　CRISPR/Cas-based　methods　mainly　depend　on　fluorophore　and　quencher　dual-labeled　ssDNA　as　the　reporter,　which　brings　in　high　cost　and　steric　hindrance.　Herein,　a　new　signal　output　format　has　been　developed.　The　method　takes　advantage　of　graphene　oxide　adsorbing　FAM-ssDNA　and　quenching　the　fluorescent　emission　while　CRISPR/Cas12a　recovering　the　fluorescent　emission　by　trans-cleaving　the　FAM-ssDNA.　Factors　affecting　the　performance　of　the　sensor　have　been　optimized,　and　it　is　shown　that　the　oligonucleotide　of　12　nt　with　the　fluorophore　modified　at　the　3　＇　end　displays　the　highest　signal　to　noise　ratio.　The　signal　output　method　has　been　combined　with　recombinase　polymerase　amplification　for　detection　of　Salmonella　and　presents　consistent　specificity　and　sensitivity　(5　x　101　copies)　as　real-time　PCR.　By　replacing　the　dual-labeled　ssDNA　reporter　with　FAM-ssDNA　and　GO,　this　signal　output　method　saves　similar　to　65%　cost.　Notably,　this　method　does　not　require　a　real-time　thermal　cycler　or　any　other　sophisticated　instrument.　Besides　pathogens,　this　easy　and　convenient　method　can　also　be　extended　to　detect　other　targets.