Rapid　and　accurate　detection　of　Salmonella　is　extremely　important　to　ensure　food　safety.　Combining　nucleic　acid　amplification　with　CRISPR/Cas12a　can　realize　sensitive　and　specific　detection　of　foodborne　pathogens.　However,　present　CRISPR/Cas-based　methods　mainly　depend　on　fluorophore　and　quencher　dual-labeled　ssDNA　as　the　reporter,　which　brings　in　high　cost　and　steric　hindrance.　Herein,　a　new　signal　output　format　has　been　developed.　The　method　takes　advantage　of　graphene　oxide　adsorbing　FAM-ssDNA　and　quenching　the　fluorescent　emission　while　CRISPR/Cas12a　recovering　the　fluorescent　emission　by　trans-cleaving　the　FAM-ssDNA.　Factors　affecting　the　performance　of　the　sensor　have　been　optimized,　and　it　is　shown　that　the　oligonucleotide　of　12　nt　with　the　fluorophore　modified　at　the　3′　end　displays　the　highest　signal　to　noise　ratio.　The　signal　output　method　has　been　combined　with　recombinase　polymerase　amplification　for　detection　of　Salmonella　and　presents　consistent　specificity　and　sensitivity　(5　×　101　copies)　as　real-time　PCR.　By　replacing　the　dual-labeled　ssDNA　reporter　with　FAM-ssDNA　and　GO,　this　signal　output　method　saves　∼65%　cost.　Notably,　this　method　does　not　require　a　real-time　thermal　cycler　or　any　other　sophisticated　instrument.　Besides　pathogens,　this　easy　and　convenient　method　can　also　be　extended　to　detect　other　targets.　©　2023　Elsevier　B.V.