Over-exploitation　of　fossil　energy　has　led　to　the　formation　of　enormous　amounts　of　oily　wastewater,　which　is　increasingly　polluting　the　environment　of　water　bodies.　The　Ti3C2Tx-modified　separation　material　is　limited　by　surface　roughness　and　surface　chemical　composition,　and　the　permeation　flux　decreases　significantly　during　continuous　testing.　In　this　study,　silver-loaded　polydopamine　nanospheres　(nano-AgPNPs)　as　antioxidants　were　prepared　by　in-situ　reduction　from　Ag+　ions　and　polydopamine　nanospheres,　and　then　they　self-assembled　with　Ti3C2Tx　nanosheets　to　obtain　hierarchical　micro-nano　structural　nano-AgPNPs　modified　Ti3C2Tx　[(AgPNPs)xT　(x　=　0.25,　0.5,　0.75,　and　1)].　Because　of　their　strong　reduction　properties,　nano-AgPNPs　antioxidants　can　effectively　protect　the　active　edges　of　Ti3C2Tx　from　dissolved　oxygen,　and　greatly　improve　the　oxidation　resistance　of　Ti3C2Tx　nanosheets,　with　no　significant　changes　in　morphology　and　physical　phase　after　long-term　placement.　(AgPNPs)xT　formed　underwater　oleophobic　interfaces　with　the　hierarchical　structure　on　polyurethane　(PU)　sponge　to　prepare　(AgPNPs)xT@PU　separation　sponges.　Among　them,　(AgPNPs)0.5T@PU　sponge　showed　the　highest　comprehensive　performance　with　separation　efficiency　higher　than　99.6%　for　various　oil-water　mixtures,　especially　high-throughput　and　permeation　flux　up　to　247,787　L　m–2　h–1　for　cyclohexane-water　mixtures.　In　addition,　nano-AgPNPs　greatly　broadened　the　layer　spacing　of　Ti3C2Tx　and　can　form　a　functionalized　interface　of　modified　Ti3C2Tx　on　polyamide　(PA)　membrane　and　provide　additional　permeation　channels,　which　enabled　the　(AgPNPs)0.5T@PA　membrane　to　have　excellent　toluene-water　emulsion　separation　efficiency　up　to　99.6%.　©　2023