Herein,　we　developed　a　platinum-copper　nano-enzyme-linked　immunosorbent　assay　(NLISA)　based　split　diagnostic　platform　for　the　ultrasensitive　detection　of　cardiac　troponin　I　(cTnI).　The　PtCu　nanozyme　synthesized　by　one-pot　synthesis　exhibited　ultra-high　peroxidase-like　activity　(35.17　U　mg-1),　which　was　about　4.5　times　higher　than　that　of　the　unmodified　Pt　nanozyme　(8.83　U　mg-　1).　Due　to　the　efficient　peroxidase-like　activity　of　the　copper-platinum　complexed　nanozyme,　transduction　and　sequential　amplification　of　cTnI　biological　signals　were　achieved　in　combination　with　a　liposome-embedded　amplification　strategy.　The　encapsulation　efficiency　was　calculated　by　introducing　a　liposomal　bilayer　model,　which　showed　that　the　introduction　of　a　single　liposomal　molecule　could　amplify　the　signal　up　to　870-fold,　thus　promising　a　high　sensitivity　test.　Notably,　the　dynamic　response　of　cTnI　was　in　the　range　of　0.1-5000　pg　mL-1　with　an　ultra-low　detection　limit　(0.048　pg　mL-1).　The　developed　NLISA　analysis　system　provides　a　new　way　to　discover　efficient　and　sensitive　alternatives　to　ELISA　kits,　which　can　meet　the　practical　needs　of　community　healthcare　testing　conditions　and　rapid　testing　in　hospitals.