A　novel　portable　biosensor　for　sensitive　and　selective　detection　of　platelet-derived　growth　factor-BB　(PDGF-BB)　had　been　developed　based　on　the　specific　recognition　between　aptamer　and　protein　using　a　personal　glucose　meter　(PGM)　as　readout.　In　the　presence　of　PDGF-BB,　the　primary　aptamer　of　PDGF-BB　which　is　bound　to　the　surface　of　streptavidin　magnespheres　paramagnetic　particles　(SA-PMPs)　reacts　quantitatively　with　invertase-functionalized　secondary　aptamer　of　PDGF-BB　to　form　a　stable　complex,　resulting　in　the　attachment　of　invertase　on　the　SA-PMPs.　Subsequently,　the　invertase　catalyzes　the　hydrolysis　of　sucrose　to　produce　a　large　amount　of　glucose　and　quantitative　readout　by　the　PGM.　The　enhanced　signal　of　the　PGM　has　a　relationship　with　the　concentration　of　PDGF-BB　in　the　range　of　1.0　x　10(-14)　M　similar　to　3.16　x　10(-12)　M,　and　the　detection　limit　is　2.9　fM.　The　proposed　portable　biosensor　had　been　successfully　applied　to　assay　the　PDGF-BB　in　saliva　samples.　(C)　2013　Elsevier　B.V.　All　rights　reserved.